Gliadin Wheat Recombinant
Gliadin is a cDNA coding for an epitope-carrying fragment of a wheat gamma-gliadin isoform, having a molecular mass of 18,616 Dalton (high proline content and the low pI are likely causes for the observed discrepancy between calculated molecular weight and the observed electrophoretic mobility of approx. 47 kDa on standard SDS-PAGE), pH 4.6. By sequence design the epitopes correspond to the deamidated neo-epitopes, which in the natural antigen are formed by transglutaminase-mediated glutamine side chain deamidation. Gliadin protein is fused to a hexa-histidine purification tag.
Accession
Applications
Western blot with monoclonal anti-hexa-His-tag antibody.
Coating concentration
0.15-0.5 ug/ml (depending on the type of ELISA plate and coating buffer).
Formulation
Gliadin is supplied in 20mM HEPES buffer pH-8.0, 200mM NaCl, and 20% glycerol.
Immunological Fuctions
Binds IgG- and IgA-type human autoantibodies associated with celiac disease.
Immunological tests
Standard ELISA test (checkerboard analysis of positive/negative sera panels); immunodot.
Purity
Greater than 94% as determined by SDS-PAGE.
Source
Storage Conditions
Store at 4oC if entire vial will be used within 2-4 weeks.
Store, frozen at -20oC for longer periods of time.
Avoid multiple freeze-thaw cycles.
