GAPDH antibody has been tested by ELISA, Western blot and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:500 ~ 10,000.
Recommended starting dilution is 1:1,000.
Mouse IgG2b heavy chain and κ light chain.
Anti-human GAPDH mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human GAPDH amino acids 1-335 purified from E. coli.
1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
GAPDH antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
12 months at -20oC. 1 month at 4oC.
For periods up to 1 month store at 4oC, for longer periods of time, store at -20oC. Prevent freeze thaw cycles.
G3PD, GAPD, MGC88685, GAPDH, Glyceraldehyde-3-Phosphate Dehydrogenase.
Mouse Anti Human Monoclonal.
