$3,990.00Catalog Number: PYT340-1mgSize: 1mg

Flt3 Ligand Mouse

Flt3 Ligand Mouse Recombinant

Flt3-Ligand Mouse Recombinant produced in E.Coli is non-glycosylated, polypeptide chain containing 163 amino acids and having a molecular mass of 18587 Dalton. Flt3-Ligand is purified by proprietary chromatographic techniques.

Accession

P49772

Amino acid sequence

MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ.

Biological Activity

The activity is determined by the ability to generate CD11c+ splenic dendritic cells in vivo in C57B1/6-12 week female mice. To obtain the desired affect, mice were treated with approximately 25ug of flt3-ligand once daily for 9 consecutive days via intraperitoneal injection.

Formulation

The FLT3L Mouse protein was lyophilized with 10mM Na2PO4 pH-7.5.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Flt-3 Ligand should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.

Quantity

$3,990.00

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