TQDCSFQHSP ISSDFAVKIR ELSDYLLQDY PVTVASNLQD EELCGGLWRL VLAQRWMERL KTVAGSKMQG LLERVNTEIH FVTKCAFQPP PSCLRFVQTN ISRLLQETSE QLVALKPWIT RQNFSRCLEL QCQPDSSTLP PPWSPRPLEA TAPTAPQP.
The ED50 as determined by the dose dependent proliferation of human OCI-AML5 cells is 0.5 to 1.0 ng/ml (Specific Activity: 2.0 x 1,000,000 - 1.0 x 1,000,000 units/mg).
The protein was lyophilized with no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Flt3-Ligand in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
HEK293 (Human Embryonic Kidney cell line).
Lyophilized Flt3-Ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Flt3-Ligand should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Fms-related tyrosine kinase 3 ligand, FLK2, STK1, CD135, Stem Cell Tyrosine Kinase 1, FLT3LG, Flt3.
