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Recombinant Flap endonuclease 1 (FEN1)

Recombinant Flap endonuclease 1 (FEN1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP008585EWUYeast1mgQuote
EP008585EWUE. coli1mgQuote
BP008585EWUBaculovirus200ugQuote
MP008585EWUMammalian Cell200ugQuote

Protein Information

SpeciesPlasmodium knowlesi (strain H)
UniProt IDB3L014
Gene NameFEN1; ORFs:PKH_030620
Protein NameFlap endonuclease 1
Region Expressed1-595
Expression Tag6xHis
Purity>90%
AA SequenceMGIKGLTKFIADAAPNAIKEIKIENLMGRVVAIDASMSLYQFIIAIRDSEQYGNLTNESG ETTSHISGLMSRSIKLMENGLKPIYVFDGAPPELKGSELEKRGEKRQKAEELLKKAKEEG NLEEIKKQSGRTVRVTKKQNEEAKKLLTLMGIPVVEAPCEAESQCAFLTKYNLAHATATE DADALVFGTKILIRNLNANASSTSQNKNKNSSKRGYILTEINLEQVLKGLNLNMNEFIDF CILCGCDYCDTIKGIGSKTAYNLIKEYNSIEKIIENIDKNKYQIPSNFRFVEARDSFINP KVLSKEEIKIDWGEPKIEELKNFLIKDYNFNEVRVTNYINRLLKARKVTTQRRLDNFFTA CTKKSTKLVNEESQIKKEVKPKRKGKKRDAPNDSSTKLNSKQNKKPKGEKESKTEKDDGD THNGNDNEEEGGEGETADMGAKDPFDTEEDEDNPSVNFFHHKSDSESGNVKKESTEQEAN ATPTGDVYSFPNGKDASGSNIHLSSNSTLHSCNNLNGDKAINESMHVVGSSAPEAGNNEI GNGDLFTSNAADCVNNNTDKTQAEIEMKKKNISFLLPYCPKNVTSVKKRKSVQRC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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