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Recombinant Flagellar hook-associated protein 1 (flgK)

Recombinant Flagellar hook-associated protein 1 (flgK) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP300287BUDYeast1mgQuote
EP300287BUDE. coli1mgQuote
BP300287BUDBaculovirus200ugQuote
MP300287BUDMammalian Cell200ugQuote

Protein Information

SpeciesBorrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
UniProt IDP70859
Gene NameflgK; Locus:BB_0181
Protein NameFlagellar hook-associated protein 1
Region Expressed1-627
Expression Tag6xHis
Purity>90%
AA SequenceMDSTFSGIEIGKRSLFAHKDAMNTVGHNLSNATKPGYSRQRVTMKTEIPLYAPQLNRAKK QGQLGQGIVVQSIDRVKDELLNTRIIEESHRLGYWTSQDKFISILEDVYNEPEDQSIRKR LNDFWESWHDLANQPQGLAERKIILERGKSFCEGIRNRFHSLERIYIMANDEIKITTDEA NNYIRNIANLNKQISKSQAMKDNPNDLMDARDLMVEKLGNIISVSIENKQDPNEFLIHAE GRHLVQGSIANEFKLEATNGPTRTRWNILWANNDKAYLKTGKLGSLLNIRDEEIKNEINE LNNIAANIIEIVNEIHEAGRGMDKKNGRSFFSQELKLTDDRGRYDTNGNGQFDSVHIFKI NSTNEIFPEEKLGFYGTLKFEATNSNEIVEIPYNAPDTVQDVINRINNSNAQVTARINSE GKLEIKAVKEQEDENITFKIKHIEDSGSFLTKYTGILNASGPEGAYDYKNIDTTDKLAPK STYSISPLKNPAAWIKVADIIDSDPSKIASGIKNPTNEISIGDNQAALRISSFGNSQIMI GKNLTLNDYFANTASNIAIKGQISEITKESQSQILKDLTDLRMSISGVNKDEELANMIEF QQAFIAASKFITVSVELIDTVINKMGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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