$176.70Catalog Number: ANTP146-500ugSize: 500ug

FLAG Antibody

FLAG peptide, Mouse Antibody

The FLAG sequence consists of eight amino acids (AspTyrLysAspAspAspAspLys) with an enterokinase-cleavage site included. Fusion protein can be detected with anti-FLAG antibody or purified with immunoaffinity chromatography. Protein elusion can be carried out under non denaturing conditions. The tag sequence is hydrophilic and significantly improves target protein detection and purification.

Clone

NYRFLAG.

Ig Subclass

Mouse IgG1/kappa.

Immunogen

Synthetic Flag peptide sequence

Note

The antibody was produced in BALB/c mice. Will recognize FLAG (DYKDDDDK) conjugated at either the amino or carboxy terminus. Can be used bound to various resins for affinity purifications.

Protein concentration

1mg/ml in PBS (after reconstitution).

Purification Method

Protein A & Ion exchange column.

Reconstitution

Reconstitute with H2O to get a 1 mg/ml concentration. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. Stability / Shelf LifeTwo years lyophilized, one month in solution at 4oC.

Shipping Conditions

Antibody is shipped lyophilized at ambient temperature.

Storage Procedures

In lyophilized form, for long periods, store at 4oC in a dry environment. After reconstitution, if not intended for use within a month, aliquot and store at -20oC.

Titer

By direct ELISA, 1:20,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Type

Mouse Antibody Monoclonal.

Quantity

$176.70

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