$148.20Catalog Number: PYT020-20ugSize: 20ug

FGF23 Human

Fibroblast Growth Factor-23 Human Recombinant

Fibroblast Growth Factor-23 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing a total of 228 amino acids and having a molecular mass of 22.5kDa.
The FGF-23 is purified by chromatographic techniques.

Accession

Q9GZV9

Amino acid sequence

MYPNASPLLG SSWGGLIHLY TATARNSYHL QIHKNGHVDG APHQTIYSAL MIRSEDAGFV VITGVMSRRY LCMDFRGNIF GSHYFDPENC RFQHQTLENG YDVYHSPQYH FLVSLGRAKR AFLPGMNPPP YSQFLSRRNE IPLIHFNTPI
PRRHTRSAED DSERDPLNVL KPRARMTPAP ASCSQELPSA EDNSPMASDP LGVVRGGRVN THAGGTGPEG CRPFAKFI.

Biological Activity

The biological activity of FGF-23 was measured in a cell proliferation assay using NIH/3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.05-0.5ug/ml in the presence of 5ug/ml of Recombinant Mouse Klotho and 10 ug/ml of heparin.

Formulation

The FGF-23 protein (0.5mg/ml) was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4.

Physical Appearance

Sterile Filtered white lyophilized powder.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized FGF-23 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized FGF-23 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution FGF-23 should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Tumor-derived hypophosphatemia-inducing factor, HYPF, ADHR, HPDR2, PHPTC, FGF23, FGF-23, Fibroblast Growth Factor-23.

Quantity

$148.20

ReviewsWrite Review