$90.00Catalog Number: PYT264-10ugSize: 10ug

FGF 1 Human

Fibroblast Growth Factor-Acidic Human Recombinant

Fibroblast Growth Factor-acidic Human Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15967 Dalton.
The FGF acidic is purified by proprietary chromatographic techniques.

Accession

P05230

Amino acid sequence

MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Biological Activity

The ED50, calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is <1ng/ml corresponding to a Specific Activity of 1,000,000IU/mg.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium phosphate pH=7.5 and 75mM NaCl.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-acidic in sterile 18MΩ-cm H2O at 4 degrees Celsius at a concentration of 0.1mg-0.25mg per 1ml. Allow sample to sit for 5 min. at 4 degrees, spin to remove precipitant.

Source

Escherichia Coli.

Stability

Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution FGF-a should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.

Synonyms

HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.

Quantity

$90.00

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