$90.00Catalog Number: PYT557-10ugSize: 10ug

FGF 2 Human (147 a.a.)

Fibroblast Growth Factor Basic 147 a.a. Human Recombinant

Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16539 Dalton.
The FGF2 is purified by proprietary chromatographic techniques.

Accession

P09038

Amino acid sequence

MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

Biological Activity

The ED50, Calculated by the dose-dependant proliferation of mouse BALB/c 3T3 cells is 0.12 ng/ml, corresponding to a specific activity of 8,333,333 Units/mg.

Formulation

The bFGF was lyophilized from a concentrated sterile solution containing 10mM sodium phosphate buffer pH=8.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized FGF-B in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized basic-FGF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution FGFb should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b.

Quantity

$90.00

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