$148.20Catalog Number: PYT288-50ugSize: 50ug

FGF 2 Bovine

Fibroblast Growth Factor-Basic Bovine Recombinant

FGF-2 Bovine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17250 Dalton. The Fibroblast Growth Factor 2 is purified by proprietary chromatographic techniques.

Accession

P03969

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ala-Ala-Gly-Ser.

Biological Activity

The ED50, measured in a mitogenic assay using quiescent NR6R-3T3 fibroblasts was found to be <0.1 ng/ml, corresponding to a specific activity of 10MUnits/mg.

Formulation

The FGF-b Bovine was lyophilized from a concentrated (1mg/ml) sterile solution containing 1%HSA.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

2. Analysis by RP-HPLC, using a calibrated solution of FGF-2 Bovine as a Reference Standard.

Purity

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized Fibroblast Growth Factor-2 Bovine Recombinant in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Fibroblast Growth Factor 2 Bovine although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution FGF-b Bovine Recombinant should be stored at 4oC between 2-7 days and for future use below -18oC.
Please prevent freeze-thaw cycles.

Synonyms

HBGH-2, HBGF-2, Prostatropin, FGF-2, FGB-b.

Quantity

$148.20

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