The sequence of the first five N-terminal amino acids was determined and was found to be Ala-His-Pro-Ile-Pro.

The protein was lyophilized from a concentrated (0.8 mg/ml) solution with 0.4 mg/ml of NaHCO₃, pH 8.

Sterile Filtered white lyophilized powder.

Bovine FGF-21 quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of FGF-21 Recombinant as a Reference Standard.

Greater than 98.0% as determined by:
(a) Analysis by Gel Filtration.
(b) Analysis by SDS-PAGE.

It is recommended to reconstitute the lyophilized Bovine FGF-21 in sterile water or 0.4% NaHCO₃, not less than 100ug/ml, which can then be further diluted to other aqueous solutions, preferably in presence of carrier protein.

Escherichia Coli.

Lyophilized FGF-21 Bovine Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18^oC. Upon reconstitution Fibroblast Growth Factor 21 should be stored at 4^oC between 2-7 days and for future use below -18^oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Fibroblast growth factor 21, FGF-21, FGF21.