$90.00Catalog Number: PYT064-5ugSize: 5ug

FGF 18 Mouse

Fibroblast Growth Factor-18 Mouse Recombinant

FGF-18 Mouse Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 180 amino acids and having a molecular mass of 21kDa.
The FGF-18 is purified by proprietary chromatographic techniques.

Accession

O89101

Amino Acid Sequence

EENVDFRIHV ENQTRARDDV SRKQLRLYQL YSRTSGKHIQ VLGRRISARG EDGDKYAQLL VETDTFGSQV RIKGKETEFY LCMNRKGKLV GKPDGTSKEC VFIEKVLENN YTALMSAKYS GWYVGFTKKG RPRKGPKTRE NQQDVHFMKR YPKGQAELQK PFKYTTVTKR SRRIRPTHPG

Biological Activity

The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is ≤ 0.5ng/ml, corresponding to a specific activity of ≥ 2.0x106 units/mg

Formulation

FGF-18 protein was lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Solubility

It is recommended to reconstitute the lyophilized FGF-8 in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli

Stability

Although lyophilized FGF 18 is stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution FGF 18 should be stored at 4oC between 2-7 days and for future use below -18oC.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please avoid freeze-thaw cycles.

Synonyms

Fibroblast growth factor 18, FGF-18, zFGF5, Fgf18, D130055P09Rik

Quantity

$90.00

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