EZCt Cells2Ct Direct qPCR Lysis Buffer
mRNA purification for gene profiling by qPCR is becoming routine for functional genomics and personalize medical studies. mRNA is sentive to RNase degradation during the many steps of isolation, making the process less reliable and time consuming. Isolating mRNA in highthroughput gene expression or targeting applications for qPCR is almost impractical.
Reagents such as Cells-to-Ct* that can be used to lyse cells in wells, and the resulting lysate could be used directly in qPCR would become the first choice. It is a very expensive 2-reagent protocol.
Lifeome scientists have developed the EZCt Cells2Ct Direct Lysis Buffer to vastly improve the process at a much affordable price. Lipid Aided RNase Inhibition (LARI) undergoes conformational changes upon contact with certain lipid molecules released from lysed cell membranes, and structurally inhibit RNase activity upon cell lysis. The inhibition is long lasting with the presence of those lipid molecules, allowing room temperature lysis and RNA stability.
- LARI Technology;
- Single Buffer;
- Room Temperature (RT) storage;
- Lysates are stable for up to 2 weeks, RT!
- 1-2-3 Protocol: wash, lyse, ready for qPCR!
- Results are in! |
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*: TM of ThermoFisher or its affiliates.
Sizes
| Cat. No. |
Volume |
Applications* |
| EZCt-1ml: |
1 ml |
20 Wells |
| EZCt-10ml: |
10 ml |
200 (2 96-well plates) |
| EZCt-100ml: |
100 ml |
2000 (10 96-well plates) |
*Est. based on 50 ul per well with less than 2000 cells of 96-well plate.
Custom size is also available upon request.
