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Recombinant Elongation factor 4 (lepA)

Recombinant Elongation factor 4 (lepA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP304207SSQYeast1mgQuote
EP304207SSQE. coli1mgQuote
BP304207SSQBaculovirus200ugQuote
MP304207SSQMammalian Cell200ugQuote

Protein Information

SpeciesSynechocystis sp. (strain PCC 6803 / Kazusa)
UniProt IDP74751
Gene NamelepA; Locus:slr0604
Protein NameElongation factor 4
Region Expressed1-603
Expression Tag6xHis
Purity>90%
AA SequenceMTDVPVSRIRNFSIIAHIDHGKSTLADRLLQVTDTVQQREMKEQFLDNMDLERERGITIK LQAARMNYKAKDGQDYVLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQGVEAQTLAN VYLALDNNLEIIPVLNKIDLPSAEPDRVAAEIEEVVGLDCSDIIQASAKAGIGVDDILEA IVQQVPPPADTVDQPLRALIFDSYYDAYRGVVVYFRVMDGQVKKGDKVRLMASDKEYVID ELGVLSPTQVQVEALHAGEVGYFAAAIKAVADARVGDTITMANSPAAEPLPGYTEANPMV FCGLFPIDADQYPDLKDALEKLKLNDAALSYEPETSSAMGFGFRCGFLGLLHMEIVQERL EREYNLDLITTAPSVIYRVTTTDEEVIEVDNPSLLPPIQKRLKVEEPFIKVEMITPETYV GTLMELCQSRRGVFKDMKFFTQTRTALIYELPLAEVVTDFFDQLKSRTKGYASMEYQLIG YRENPLVKLDILVNGDGVDALAMIVHRDKAYYVGRAMVSKLKELIPRHQFKVPIQAAIGA KVIASEHIPALRKDVLAKCYGGDISRKKKLLQKQAKGKKRMKAIGTVDVPQEAFMAVLKL DPQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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