Request QuoteCatalog Number: xP301132MLWSize: 0.2-1mg

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Recombinant Elongation factor 4 (lepA)

Recombinant Elongation factor 4 (lepA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP301132MLWYeast1mgQuote
EP301132MLWE. coli1mgQuote
BP301132MLWBaculovirus200ugQuote
MP301132MLWMammalian Cell200ugQuote

Protein Information

SpeciesMycoplasma pneumoniae (strain ATCC 29342 / M129)
UniProt IDP75498
Gene NamelepA; Locus:MPN_279; ORFs:MP556
Protein NameElongation factor 4
Region Expressed1-598
Expression Tag6xHis
Purity>90%
AA SequenceMEQQKIRNFSIIAHIDHGKSTLSDRLIERSIGFEKRLLQAQMLDTMAIERERGITIKLNA VQLKMAQGNQQYLFHLVDTPGHVDFTYEVSRSLAACEGVLLLVDATQGIQAQTISNTYLA LENNLEIIPVINKVDMESADVEKTKQAFHQLLGVDPNTIPLVSAKTGLGIDQLITTIIEK VPPPKGDESKPLKALLFDSYYDPYKGVVCFIRIFEGSLKLNDKIRFARSNSVYQIVELGI KNPFFEKQDVLKAGEIGWFSAGIKKLRDVTVGDTIVHAEDTTTPPLPGYKKVLPMIYCGL YPIDNNDYQNLKMAMEKIILSDVALEYEYETSQALGFGVRCGFLGLLHMDVIKERLEREY NLKLISAPPSVRYKVLLTNGEELELDNPSLLPERSRIKSISEPFVRVYIDLPDHYLGTVI DLCQNFRGQYEKLEEIDIDRKRLVYLMPLGEIIYSFFDKLKSITKGYASLNYEFDQYQVS QLAKVEIMLNKQKVDALSFIAHHDFAFQRAKKFCVKLKELIPKHLFEIPIQATIGSKVIA RETIKAVRKDVTAKLYGGDVTRKKKLLEKQKEGKKRLKAIGSVELPQELFSHLLKDED
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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