Request QuoteCatalog Number: xP503869RMNSize: 0.2-1mg

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Recombinant Elongation factor 4 (lepA)

Recombinant Elongation factor 4 (lepA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP503869RMNYeast1mgQuote
EP503869RMNE. coli1mgQuote
BP503869RMNBaculovirus200ugQuote
MP503869RMNMammalian Cell200ugQuote

Protein Information

SpeciesRickettsia africae (strain ESF-5)
UniProt IDC3PMX3
Gene NamelepA; Locus:RAF_ORF0344
Protein NameElongation factor 4
Region Expressed1-600
Expression Tag6xHis
Purity>90%
AA SequenceMNHQKYIRNFSIIAHIDHGKSTLADRLIEHCGGLQAREMSQQVLDSMDIEKERGITIKAQ TVRLVYKAKDGNTYYLNLMDTPGHVDFAYEVSRSLAACEGSLLVVDSTQGVAAQTLANVY QAIENDHEIVLVLNKLDLPASEPEQVKQQIEDIIGIDTSDAVLISAKSGIGIDLVLEAIV NKLPPPKESSSDILKALLVDSWYDQYLGVVILVRVIDGYLRKNMRIKMMATNSVYTVENV GYFTPKKHISDVLHAGEIGFFTAAIKQVADCKVGDTITDEKKPCEQALPGFKPNLPVVFC GLYPTDSSEFEHLKDSLAKLRLNDASFEYEMESSSALGVGFRCGFLGLLHLEIIQERLSR EFDLDLITTAPSVVYKIHMQDGENLEIHNPADLPNLQKIESMEEPWIKATIMVPDEFLGA VLSLCTEKRGMQLDHSYIANRAKIIYKLPLNEIVYDFYDRLKSCSKGYASFEWQMDVYEP SELVKLGILVNAEVVDALSTIVHRSRAEQRGRALCVRLKDLIPRQQIDIAIQASIGSRII ARETIKALRKDVLSKCYGGDISRKRKLLEKQKAGKKRMRQYGNIEIPQSAFIAALKIGDE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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