NSNTGCPPSY DGYCLNGGVC MYVESVDRYV CNCVIGYIGE RCQHRDLRWW KLR.
The ED50 as calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.1ng/ml, corresponding to a specific activity of > 10,000,000 units/mg.
Rat EGF was lyophilized from a 0.2um filtered concentrated (1.0mg/ml) solution in PBS, pH 7.4.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized Rat EGF in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized Rat EGF although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Rat EGF should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
