$90.00Catalog Number: PYT217-100ugSize: 100ug

EGF Human

Epidermal Growth Factor Human Recombinant

Epidermal Growth Factor Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 53 amino acids and having a molecular mass of 6222 Dalton.
The EGF is purified by proprietary chromatographic techniques.

Accession

Q6QBS2

Amino acid sequence

NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR.

Biological Activity

The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 1 x 10,000,000 Units/mg.

Formulation

The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM Phosphate buffer pH-7.4.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Protein content

EGF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.

Purity

Greater than 98.0% as determined by:
(a) Analysis by SEC-HPLC.
(b) Analysis by SDS-PAGE.

References

Title:Activation of the Hedgehog pathway in pilocytic astrocytomas.
Publication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010
Link:http://neurooncology.oxfordjournals.org/content/12/8/790.full

Solubility

It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Source

Escherichia Coli.

Stability

Lyophilized Epidermal Growth Factor Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below
-18oC. Upon reconstitution EGF should be stored at 4oC between 2-7 days and for future use below -18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

Urogastrone, URG, EGF.

Quantity

$90.00

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