Request QuoteCatalog Number: xP010115DILSize: 0.2-1mg

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Recombinant E3 ubiquitin-protein ligase HACE1 (hace1)

Recombinant E3 ubiquitin-protein ligase HACE1 (hace1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP010115DILYeast1mgQuote
EP010115DILE. coli1mgQuote
BP010115DILBaculovirus200ugQuote
MP010115DILMammalian Cell200ugQuote

Protein Information

SpeciesDanio rerio (Zebrafish) (Brachydanio rerio)
UniProt IDF8W2M1
Gene Namehace1
Protein NameE3 ubiquitin-protein ligase HACE1
Region Expressed1-904
Expression Tag6xHis
Purity>90%
AA SequenceMERAMEHLNVQLNRLTRSLRRARTVELPEDSETAVYTLMPMVMADQHRSVSELLLNSKFD VNYAFGRVKRSLLHIAANCGSVECLVLLLKRGANPNYQDISGCTPLHLAARNGQKKCMGR LLEYNADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTNVDVEDAMGQTALHVACQNGH KTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDRNGVTPLDLC VQGGYGETCEILIQHHGRLFQTLIQMTQNDDIKENMLRQVLEHVSQQNDSNYQRILTSLA EVATTNGHKLLSLSSNFEVQTKSLLRIIRIFCHVFCLGPSSPNNGNDMGYNGNKTPRSQV FKPLELLWHSLDEWLVLISTELEKEITDTTRSSSGNDIASLFLKKQEVDHSVSSENPQLL LDASSVMKTPEVYADGQDVISMIANRLSAVIQAFYMCCSCQMPHGMTSPRFIEFVCKHDE VLKCFVTRNPKIIFNHFHFLLECPELMSRFMHIIKGQPFKDRCEWFYEHLLAGQPDSDMV HRPVNENDILLVHRDSLFRSSCEVVSKSSNEKLKQGIAVRFHGEEGMGQGVVREWFDILS NEIINPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALYHRQLVNIYFTRS FYKHILGIPVSYQDVSSIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGTMEEVPLKP GGTTIQVTQDNKEEYVQLVTELRMTRAIQPQINAFLQGFHTFIPPSLIQLFDEYELELLL SGMPEIDVMDWKRNTEYTSGYDLQEPVIQWFWEVVENLTQEERVLLLQFVTGSSRVPHGG FAFLMGGSGLQKFTVAAVPYTSNLLPTSSTCINMLKLPEYPSKDVLRDRLLVALHCGSYG YTMA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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