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Recombinant E3 ubiquitin-protein ligase HACE1 (HACE1)

Recombinant E3 ubiquitin-protein ligase HACE1 (HACE1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP010115BOYeast1mgQuote
EP010115BOE. coli1mgQuote
BP010115BOBaculovirus200ugQuote
MP010115BOMammalian Cell200ugQuote

Protein Information

SpeciesBos taurus (Bovine)
UniProt IDF1N6G5
Gene NameHACE1
Protein NameE3 ubiquitin-protein ligase HACE1
Region Expressed1-909
Expression Tag6xHis
Purity>90%
AA SequenceMERAMEQLNRLTRSLRRARTVELPDDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYA FGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEY SADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVSDVDVEDAMGQTALHVACQNGHKTTV QCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLSDKNGVTPLDLCVQGG YGETCEVLIQYHPRLFQTIIQMTQNEDLRENMLRQVLEHLSQQSESQYLKILTSLAEVAT TNGHKLLSISSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPL ELLWHSLDEWLVLIATELMKNKKDSTDITSILLKQKGQDQDGTSIPPFEPPGPGSYENLS TGTGESKPDVLGGKQEASADCQDVISMTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFV CKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQP DSDMVHRPVNENDILLVHRDSIFRSSCEVVSKANCAKLKQGIAVRFHGEEGMGQGVVREW FDILSNEIVNPDYALFTQSADGTTFQPNSNSSVNPDHLNYFRFAGQILGLALNHRQLVNI YFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEE VPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYE LELLLSGMPEIDVSDWIKNTEYTSGYEREDPVIQWFWEVVEDITPEERVLLLQFVTGSSR VPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALH CGSYGYTMA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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