DnaJ (Hsp40) Homolog, Subfamily B, Member 8 Human Recombinant
DNAJB8 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 252 amino acids (1-232 a.a.) and having a molecular mass of 27.8kDa. DNAJB8 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Accession
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MANYYEVLGV QASASPEDIK KAYRKLALRW HPDKNPDNKE EAEKKFKLVS EAYEVLSDSK KRSLYDRAGC DSWRAGGGAS TPYHSPFDTG YTFRNPEDIF REFFGGLDPF SFEFWDSPFN SDRGGRGHGL RGAFSAGFGE FPAFMEAFSS FNMLGCSGGS HTTFSSTSFG GSSSGSSGFK SVMSSTEMIN GHKVTTKRIV ENGQERVEVE EDGQLKSVTV NGKEQLKWMD SK.
Formulation
The DNAJB8 solution (0.25mg/ml) 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT.
Physical Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Source
Stability
Store at 4oC if entire vial will be used within 2-4 weeks. Store, frozen at -20oC for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Synonyms
DnaJ homolog subfamily B member 8, DNAJB8, DJ6.