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Recombinant DNA primase (repB)

Recombinant DNA primase (repB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340852TOAYeast1mgQuote
EP340852TOAE. coli1mgQuote
BP340852TOABaculovirus200ugQuote
MP340852TOAMammalian Cell200ugQuote

Protein Information

SpeciesThiobacillus ferrooxidans (Acidithiobacillus ferrooxidans)
UniProt IDP27186
Gene NamerepB
Protein NameDNA primase
Region Expressed1-352
Expression Tag6xHis
Purity>90%
AA SequenceMKSEQLKQFERYAEAVGAERYRVTSIKMQADGRKQTFILDKKDGITRGFTPQEIEQRTPE MQRLQRRGENLYYTPLSDKKHHILIDDMNREKLERLIKDGYQPAAVLESSPGNYQAIITV SKLGTAHDKDVGNRLSDALNREYGDPKLSGAIHPHRAPGYENRKPKHQREDGSYPEVRLL KAERRECVKALALSSQIDAEYQRQAALKAQQPERTKAKPALELAAASGSAIDAYQRHYRD VLKRQRGGEVDLSRVDSMIAVRMRVTGHDQAAIEGAIRQCAPATRQKDEGRDWNDYAQRT ARYAYSAAQPQAADLGKYRQQWEKLEGREPVRQQEQAKAQKIERDNSPGMSL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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