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Recombinant DNA primase large subunit (Prim2)

Recombinant DNA primase large subunit (Prim2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP018681RAYeast1mgQuote
EP018681RAE. coli1mgQuote
BP018681RABaculovirus200ugQuote
MP018681RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDO89044
Gene NamePrim2
Protein NameDNA primase large subunit
Region Expressed1-507
Expression Tag6xHis
Purity>90%
AA SequenceMQFSGRTRKKLRLAGDQRNACYPHSLQFYLQPPTENISLTEFESLAFDRVKLLKAIENLG VSYVKGTEQYQSKLEAEIRKLKFSYRENLEDEYEPRRRDHISHFILRLAYCQSEDLRRWF IQQEMDLLRFRFSILPKDKVQSFLKDTHLHFEAISDEEKTLREQDIMASSPSLSGVRWES ESVYKVPFADALDLFRGRKVYLEDGFAYVPLKDIVAIILNEFRATLSKALALTARSLPAV QSDERLQPLLSHLSHSYTGQDYSTQKSTGKISLDQIDSLSTKSFPPCMRQLHKALRENHH LRHGGRMQYGLFLKGIGLTLEQALQFWKQEFIKGKMDPDKFDKGYSYNIRHSFGKEGKRT DYTPFSCMKIILTNPPSQGDFHGCPFRHSDAELLKQKMQTYKIPASGISQILDLVKGNHY QVACQKYFEMTHNVDDCGFSLNHPNQFFFESQRILTGGKDIKKEASHPETPQHKPSTQKT KDATSALASLDSSLEMDLEGLEDYFSK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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