Request QuoteCatalog Number: xP340715SVGSize: 0.2-1mg

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Recombinant DNA polymerase IV (POL4)

Recombinant DNA polymerase IV (POL4) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP340715SVGYeast1mgQuote
EP340715SVGE. coli1mgQuote
BP340715SVGBaculovirus200ugQuote
MP340715SVGMammalian Cell200ugQuote

Protein Information

SpeciesSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
UniProt IDP25615
Gene NamePOL4; aka: POLX; Locus:YCR014C; ORFs:YCR14C
Protein NameDNA polymerase IV
Region Expressed2-582
Expression Tag6xHis
Purity>90%
AA SequenceSLKGKFFAFLPNPNTSSNKFFKSILEKKGATIVSSIQNCLQSSRKEVVILIEDSFVDSDM HLTQKDIFQREAGLNDVDEFLGKIEQSGIQCVKTSCITKWVQNDKFAFQKDDLIKFQPSI IVISDNADDGQSSTDKESEISTDVESERNDDSNNKDMIQASKPLKRLLQGDKGRASLVTD KTKYKNNELIIGALKRLTKKYEIEGEKFRARSYRLAKQSMENCDFNVRSGEEAHTKLRNI GPSIAKKIQVILDTGVLPGLNDSVGLEDKLKYFKNCYGIGSEIAKRWNLLNFESFCVAAK KDPEEFVSDWTILFGWSYYDDWLCKMSRNECFTHLKKVQKALRGIDPECQVELQGSYNRG YSKCGDIDLLFFKPFCNDTTELAKIMETLCIKLYKDGYIHCFLQLTPNLEKLFLKRIVER FRTAKIVGYGERKRWYSSEIIKKFFMGVKLSPRELEELKEMKNDEGTLLIEEEEEEETKL KPIDQYMSLNAKDGNYCRRLDFFCCKWDELGAGRIHYTGSKEYNRWIRILAAQKGFKLTQ HGLFRNNILLESFNERRIFELLNLKYAEPEHRNIEWEKKTA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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