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Recombinant DNA double-strand break repair Rad50 ATPase (rad50)

Recombinant DNA double-strand break repair Rad50 ATPase (rad50) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520576HTUYeast1mgQuote
EP520576HTUE. coli1mgQuote
BP520576HTUBaculovirus200ugQuote
MP520576HTUMammalian Cell200ugQuote

Protein Information

SpeciesHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
UniProt IDD4GUK1
Gene Namerad50; Locus:HVO_0854
Protein NameDNA double-strand break repair Rad50 ATPase
Region Expressed1-893
Expression Tag6xHis
Purity>90%
AA SequenceMRFTRIAIRNFKPYEDAELDLRDGVTVIHGVNGSGKSSLLEACFFALYGSKALAGTLEDV VTTGADDAEITLEFVHDGGEYRIDRRVRVSGDRATTAKCVLDGPEGTVEGARDVRRHVAS LLRMDAEAFVNCAYVQQGEVNKLINATPSQRQDMIDDLLQLGKLETYRERAGNARLGVED VLTKKRSVLEDVESQIEAKEDADLHATLNALESELDSLDEEISNYESQRDKAKSALDAAE ATLDEHAEKRERLDEIESAIEDLTAKISADETKRDDLSERVRELDAAADELESDIDDALA RADLDDASDEAIADAREALSARESELRDDLSEARTRAQAFETQAERLRERADDLESRAEE KREAAEVDAETAAEAERELESFRETRAELRSALDDAEARFEDAPVELGEAADLLEERREA RSEARESLAETETELKNARERLAEAERLRDAGKCPECGQPVEGSPHVDAISEREAAVESL EADRESLESAVESRSAAVEAAEALVEVETRASSRRDRLDLVDERIADREETVESRREAAE EKREAAAELDSEAEEKREAATTQAERAEEVAETVDSLESDLDTLDDRRDRLDRVESLVDT RAEKIDARDRLREKRETLAEVNRERREHLRERRERRDELRETVDEEAVEGAKQRKKNAAA YLERVEDEVLPELREKRDDLQSRVGGVEAELEQLDDLRERRDHLAERVDALESVHDEVST LESTYGDLRAELRQRNVEVLERMLNETFDLVYANDAYSRIRLDGEYGLTVFQKDGTALEP EQLSGGERALFNLSLRCAIYRLLAEGIDGAAPLPPLILDEPTVFLDGGHVSRLVDLVEDM QSRGVKQILIVSHDEDLVGAADDLVRVEKNPTTNRSTVERTDAPIVEGALADD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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