Request QuoteCatalog Number: xP305039SGCSize: 0.2-1mg

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Recombinant DNA-directed RNA polymerase subunit alpha (rpoA)

Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP305039SGCYeast1mgQuote
EP305039SGCE. coli1mgQuote
BP305039SGCBaculovirus200ugQuote
MP305039SGCMammalian Cell200ugQuote

Protein Information

SpeciesShewanella sp. (strain DB6705)
UniProt IDP74963
Gene NamerpoA
Protein NameDNA-directed RNA polymerase subunit alpha
Region Expressed1-328
Expression Tag6xHis
Purity>90%
AA SequenceMQGSVTEFLRPRLVDIEQISSTRAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVE IDGVLHEYSSIEGVQEDVLEILLNLKGLAIKLEGKDEALLTLTKSGTGPVLAGDITHDGD VEIINPEHVIRNLTGQADISMRIRVAKGRGYVPASTRIQSDDEERPIGRLLVDAAFSPVS RIAYNVESARVEQRTNLDKLVIDMETNGTLDPEEAIRRAATILAEQLDAFVDLRDVTEPE QKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLT EIKDVLASRGLSLGMRLENWPPASIADE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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