Request QuoteCatalog Number: xP523705GHGSize: 0.2-1mg

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Recombinant DNA-directed RNA polymerase subunit alpha (rpoA)

Recombinant DNA-directed RNA polymerase subunit alpha (rpoA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP523705GHGYeast1mgQuote
EP523705GHGE. coli1mgQuote
BP523705GHGBaculovirus200ugQuote
MP523705GHGMammalian Cell200ugQuote

Protein Information

SpeciesGuillardia theta (Cryptomonas phi)
UniProt IDO46914
Gene NamerpoA
Protein NameDNA-directed RNA polymerase subunit alpha
Region Expressed1-318
Expression Tag6xHis
Purity>90%
AA SequenceMTQFEIECLDSKVYNARDHWSRFVIDPLKPGQGTTLGNALRRTLLSELEGLAITAVRIAG VSHEFSTINGIREDVLEILLNLKEIVFSGQLQESTIGRLTVQGPAIVTARSFELPPEIKL IDPEQYIATICGNNTLEMEFKIEVGSGYKIVEKDNSTEHFEFLQVDSVFMPVKKVNFRIE ETRGENNILNEQLILDIWTNGSISAKEAISNAADNLINLFSPLKTIDGESETDYETNETS HINQILIEELQLSVRAYNCLKRGCAHIHSVADLLDYSQEDLIEIKNFGQKSAEEVIDALQ KRLGINLPKEKTTKIYNK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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