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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP386807BPUYeast1mgQuote
EP386807BPUE. coli1mgQuote
BP386807BPUBaculovirus200ugQuote
MP386807BPUMammalian Cell200ugQuote

Protein Information

SpeciesBurkholderia pseudomallei (strain 668)
UniProt IDA3N5Z1
Gene NamemiaB; Locus:BURPS668_0710
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-457
Expression Tag6xHis
Purity>90%
AA SequenceMTKKVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVF SDLGRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRE SGRAQVDITFPEIEKFDHLPPARVEGPSAFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDD VLTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTT SHPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRP NLSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELK LKRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAA HPRLIGQMIDVKINHAYPHSLRGELVLAHGDASAATH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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