Request QuoteCatalog Number: xP386749AUFSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP386749AUFYeast1mgQuote
EP386749AUFE. coli1mgQuote
BP386749AUFBaculovirus200ugQuote
MP386749AUFMammalian Cell200ugQuote

Protein Information

SpeciesActinobacillus pleuropneumoniae serotype 5b (strain L20)
UniProt IDA3N1X3
Gene NamemiaB; Locus:APL_1325
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-475
Expression Tag6xHis
Purity>90%
AA SequenceMAKLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFS QLGRWKNWKKDKPDLIIGVGGCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGG DRAIVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQ ISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKER LARLQQRINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFQGTPDMIGK FVDIKITDVYTNSLRGDVVRTEDEMGLRVVESAASVIARTRKEDDLGVGKYVVNL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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