Request QuoteCatalog Number: xP389027STBSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP389027STBYeast1mgQuote
EP389027STBE. coli1mgQuote
BP389027STBBaculovirus200ugQuote
MP389027STBMammalian Cell200ugQuote

Protein Information

SpeciesSaccharopolyspora erythraea (strain NRRL 23338)
UniProt IDA4FAJ1
Gene NamemiaB; Locus:SACE_1748
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-491
Expression Tag6xHis
Purity>90%
AA SequenceMTRSYQIRTYGCQMNVHDSERLAGMLEDAGYVRAGTDVDPDVVVFNTCAVRENADNRLYG NLGHLRPAKDRNPGMQIAVGGCLAQKDRGEIVKRAPWVDVVFGTHNLGSLPTLLERARHN EEAEVEILESLDVFPSTLPARRESAYSGWVSVSVGCNNTCTFCIVPALRGKEKDRRPGEI LSEVQALVSEGVLEVTLLGQNVNAYGVEFGDRFAFGKLLRSCGEIEGLERVRFTSPHPRD FTDDVIAAMAETPNVCHQLHMPLQSGSDRVLKQMRRSYRSQRYLDILRKVREAMPDAAIT TDIIVGFPGETEEDFEATMQVVREARFASAFTFQYSKRPGTPAAEMEGQLPKEVVQQRYD RLIELQNQISWDVGKELVGRSVELLVAEGEGRKDTETHRLSGRARDGRLVHFTPVGAVDG QVRPGDVVHTTITRAAPHHLVADSEITAHRRTRAGDHWEEGVRPKTSGVSLGLPSFGAPP AQPAAQQGCAC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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