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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP406060FDUYeast1mgQuote
EP406060FDUE. coli1mgQuote
BP406060FDUBaculovirus200ugQuote
MP406060FDUMammalian Cell200ugQuote

Protein Information

SpeciesFlavobacterium psychrophilum (strain JIP02/86 / ATCC 49511)
UniProt IDA6H119
Gene NamemiaB; Locus:FP1979
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-481
Expression Tag6xHis
Purity>90%
AA SequenceMEKIIEESKQGGSLILENKPENTKKLFIESYGCAMNFSDSEIVASILSGNGYNTTNVLEE ADLVLVNTCSIRDKAEQTIRKRLEKYNAVKRINPKMKVGVLGCMAERLKDKFLEEEKIVD LVVGPDAYKDLPNLLNEVEEGRDAINVILSKDETYGDISPVRLMSNGITALVAITRGCDN MCTFCVVPFTRGRERSREPQSIMAEIQDLWHKGFKEITLLGQNVDSYLWYGGGLKKDFTN ASEIQKATAVDFDQLLEMVAVGFPKMRIRFSTSNPQDMHESILHVMAKHSNICKHIHLPV QSGSNRILKEMNRLHTREEYMILIDKIRAIIPNASISQDMIAGFPTETEEDHQDTISLMQ YVKYNFGYMYSYSERPGTLAGRKMKDDVSDEIKARRLQEIVDLQQKHAWWRSEDFIGQTV EVLVEKVSKKSTEEFSGRNSQSITVVFPKEHYKIGDFVNVKIKSCTSGTLKGEAVGYSEM N
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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