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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP374360AVLYeast1mgQuote
EP374360AVLE. coli1mgQuote
BP374360AVLBaculovirus200ugQuote
MP374360AVLMammalian Cell200ugQuote

Protein Information

SpeciesAzoarcus sp. (strain BH72)
UniProt IDA1K3J0
Gene NamemiaB; Locus:azo0778
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-449
Expression Tag6xHis
Purity>90%
AA SequenceMKKLYIRTFGCQMNEYDSEKMADVLGAGEGIAKTDNPEEADVILFNTCSVREKAQEKVFH DLGRVKHLKQLKPDLIIGVGGCVASQEGDAIVARAPYVDVVFGPQTLHRLPALIAARKQS GRSQVDISFPEIEKFDAMPPARVEGASAFVSIMEGCSKYCTFCVVPYTRGEEISRPLEDV LAEIAGLAEQGVKEVTLLGQNVNAWRGEIVRDAGEEGDFAFLLECVAEIPGIERIRYTTS HPREMTQRLFDAYVKIPKLVSQLHLPVQSGSDRILAAMKRGYSVLEFKSIVRKLRAARPD LSLSSDFIVGFPGETEEDFEKTMKLIDEVGFDGSFSFVYSARPGTPAADLEDPVPQETKL AWLARLQKRIDEQAQAVSQSMVGSAQRILVEGVSRKSAEELMGRTDNNRVVNFPAPSPHR DRLVGQFIEVRITSALPHSLRGEILTRES
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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