Request QuoteCatalog Number: xP374723YAKSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP374723YAKYeast1mgQuote
EP374723YAKE. coli1mgQuote
BP374723YAKBaculovirus200ugQuote
MP374723YAKMammalian Cell200ugQuote

Protein Information

SpeciesYersinia enterocolitica serotype O:8 / biotype 1B (strain 8081)
UniProt IDA1JQA3
Gene NamemiaB; Locus:YE2985
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMTKKLHIKTWGCQMNEYDSSKMADLLASTHGYQLTEIPEEADLLLLNTCSIREKAQEKVF SLLGQWKLLKEKNPELIIGVGGCVASQEGEHLRQRAPCVDVIFGPQTLHRLPEMINHVQG THSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDD ILFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSH PIEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDI QISSDFIIGFPGETQQDFEQTMKLVADVRFDTSYSFIYSPRPGTPAADLPDDVSEEEKKQ RLHILQQRITQQAMEISREMVGTVQRILVEGTSRKNVMELAGRTENNRVVNFEGTPEMIG KFVDVEIVDVYASSLRGILLRTEDQMDLRIHESPQSVIARTRKENELGVGLYQP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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