Request QuoteCatalog Number: xP395823CVXSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP395823CVXYeast1mgQuote
EP395823CVXE. coli1mgQuote
BP395823CVXBaculovirus200ugQuote
MP395823CVXMammalian Cell200ugQuote

Protein Information

SpeciesCaldicellulosiruptor saccharolyticus (strain ATCC 43494 / DSM 8903)
UniProt IDA4XL48
Gene NamemiaB; Locus:Csac_2048
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-471
Expression Tag6xHis
Purity>90%
AA SequenceMENRGIYYIDFGTQAQFIEEIEKMNSEYYFKNGKNKTYHIITYGCQMNVHDSEKLAGMLN AMGYVETQNLEEADLIIFNTCAVREHAESRVYGNIGPLKRLKDKKPELIIGVCGCMPQQL EVAQKLAKVFPFLDIIFGTKSLHKFPQLLYKAITTKKTVIDVAEDEDVVVEGIPTARREG VSAFVNIIYGCNNFCSYCIVPYVRGRERSRRPEEIIFEIQQLAANGVKEVTLLGQNVNSY GKDLPDGIPFYKLLEKVNAIEGIERIRFVTSHPKDLSDELIFAMRDLEKVCEHIHLPVQS GSTRILRQMNRHYTKEDYLRLVEKLKENIPDIAITTDIIVGFPGETEEDFEDTLDVVRKV EFDSAYTFIYSKRKGTKAAQMPNQVPDEVKHERFQRLVKLVEEIALKKNQQMLGKVCEIL IDGYSKRNNLLEGRTRTNKVVNVKCSDEFMYKFVNVKILEASRHWLYGEVI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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