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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP395781STDYeast1mgQuote
EP395781STDE. coli1mgQuote
BP395781STDBaculovirus200ugQuote
MP395781STDMammalian Cell200ugQuote

Protein Information

SpeciesSalinispora tropica (strain ATCC BAA-916 / DSM 44818 / CNB-440)
UniProt IDA4X4V8
Gene NamemiaB; Locus:Strop_1442
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-499
Expression Tag6xHis
Purity>90%
AA SequenceMTTAAASSPRTYQVRTYGCQMNVHDSERIAGLLEQAGYLRAGAADDTPDVVVFNTCAVRE NADNRLYGNLGQLRPRKDQHPEMQIAVGGCLAQKDRSEIVRRAPWVDVVFGTHNIGSLPV LLERARHNAAAEVEILESLEVFPSTLPTRRESTYAGWVSISVGCNNTCTFCIVPALRGKE KDRRPGEILSEVRSLVDEGVLEVTLLGQNVNSYGIEFGDRYAFGKLLRACGDIDGLERVR FTSPHPKDFTDDVIAAMAETPNVCPSLHMPLQSGSDDMLRAMRRSYRSERYLGIIEKVRA AMPDAAITTDIIVGFPGETEADFERTLDVVRAARFSSAFTFQYSKRPGTPAATMADQLPK AVVQERYERLIDCVEEITWAENRRLLGKTVEVLVAVGEGRKDERTGRLSGRARDGRLVHF DAGSLAGQVRPGDIVQTVVTYAAPHHLNADGEPLAHRRTRAGDAAEAGQRPRTAGVSLGL PTVGAPPPMPPAASSACSC
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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