Request QuoteCatalog Number: xP372677CWOSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP372677CWOYeast1mgQuote
EP372677CWOE. coli1mgQuote
BP372677CWOBaculovirus200ugQuote
MP372677CWOMammalian Cell200ugQuote

Protein Information

SpeciesChlorobium phaeobacteroides (strain DSM 266)
UniProt IDA1BIX2
Gene NamemiaB; Locus:Cpha266_2361
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMPRARRKFYIHTFGCQMNQADSGIITALLEKEGYQQAASEEEAGIIMLNTCAVRENAVER ISHYLQHVKGFKRKCPELIVGLTGCIPQYRREELFTVFPVIDFLAGPDTYRVLPALIAEA GDSRSARLEFNAFETYDGVRQARTQSLNAFVPIIRGCNNMCAFCVVPFTRGRERSHPFES VLDEVRALADDGCREITLLGQNVNSYHDPASGADFSRLLDAVSREAPETRIRFTTSHPKD MSHSLVETMALRPNICNHLHLPVQSGSTRMLARMNRGHDIEEYRNKIELLRKWIPGISLS TDLIAGFCGESEADHDQTLELMREVRFDSVFMFYYSVRQGTLAARTMPDDVPEEVKKQRL QEIIDLQNGISAELLGLAPGSVVEVLVESESRRSSDQLMGRTDGNRVVVFDRGIHQPGDL VRVMITGSTSATLFGQSAENLQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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