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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP367646MOPYeast1mgQuote
EP367646MOPE. coli1mgQuote
BP367646MOPBaculovirus200ugQuote
MP367646MOPMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium ulcerans (strain Agy99)
UniProt IDA0PT87
Gene NamemiaB; Locus:MUL_3378
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-532
Expression Tag6xHis
Purity>90%
AA SequenceMTSTVAHGAGSAGPADDVEPMSARTYQVRTYGCQMNVHDSERMAGLLEAAGYRRAAEGTD ADVVVFNTCAVRENADNKLYGNLSHLAPRKRTSPDMQIAVGGCLAQKDRDALLRKAPWVD VVFGTHNIGSLPALLDRARHNRVAQVEIAEALQQFPSSLPSARESAYAAWVSISVGCNNT CTFCIVPSLRGKEIDRSPADILAEVQSLVDTGVVEITLLGQNVNAYGVSFADPALPRNRG AFAELLRACGDIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGSDRVLRA MRRSYRAERYLGIIERVRAAMPHAAITTDLIVGFPGETEQDFAATLDVVRQARFSAAFTF QYSKRPGTPAAELDGQLPKAVVQERYERLVELQEQISLEGNRAIVGQRVELLVATGEGRK DTLTARMSGRARDGRLVHFRAGDGPVRPGDIVTVEVTDAAPHHLIADGGILAHRRTRAGD AHADGQTVRGIGLGMPGIGRPVVPVAAEATSCGSAGGCGSADGAGSSAGDPQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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