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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP369398GEQYeast1mgQuote
EP369398GEQE. coli1mgQuote
BP369398GEQBaculovirus200ugQuote
MP369398GEQMammalian Cell200ugQuote

Protein Information

SpeciesGramella forsetii (strain KT0803)
UniProt IDA0M7D3
Gene NamemiaB; Locus:GFO_3590
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-481
Expression Tag6xHis
Purity>90%
AA SequenceMEKIIDEKKQGNSLVLEPKAKNTRKLFIESYGCAMNFSDSEIVASILSKEGFNTTQNLEE ADLVLVNTCSIREKAEQTVRKRLEKYNAVKRINPGMKVGVLGCMAERLKDKFLEEEKIVD LVVGPDAYKDLPNLINEVEEGRDAINVILSKEETYGDISPVRLQSNGVSAFVSITRGCDN MCTFCVVPFTRGRERSRDPQSIVEEVNDLAAKGYKEITLLGQNVDSYLWYGGGLKKDFKN ATEIQKATATNFAALLKLVAEAQPKMRIRFSTSNPQDMTLDVIEAMAAYRNICNYIHLPV QSGSDRILKKMNRLHTREEYFTLIDNIKKMIPNCGISHDLITGFPTETEEDHQDTLSLME YVKYDFGYMFTYSERPGTTAERKLEDDIPEETKKRRLQEIVDLQQKHSKQNTNSVIGTTV EVLIEKESKKSNKHWSGRNERNTVTVFSKENYQIGDFVKVKIHDCTSATLIGEPIGLSDN N
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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