Request QuoteCatalog Number: xP370592AUASize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP370592AUAYeast1mgQuote
EP370592AUAE. coli1mgQuote
BP370592AUABaculovirus200ugQuote
MP370592AUAMammalian Cell200ugQuote

Protein Information

SpeciesAcidothermus cellulolyticus (strain ATCC 43068 / 11B)
UniProt IDA0LV00
Gene NamemiaB; Locus:Acel_1488
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-497
Expression Tag6xHis
Purity>90%
AA SequenceMTLLDSDSRQSAEVLPAAGPAPDRPRTYQVRTFGCQMNMHDSERIAGLLEAAGYVRAADD EPADVVVFNTCAVRENADNRLYGNLGLLLPVKKAKPDMQIAVGGCLAQKDKGDIVRRAPW VDVVFGTHNIEALPVLLERARIAREAQVEIREALEVFPSTLPSRRESVYAAWVAISVGCN NTCTFCIVPSLRGRERDRRPGDILREIQTLVADGVLEVTLLGQNVNSYGVDFGDRQAFAK LLRACGTIDGLERVRFTSPHPRDFTDDVIAAMAETPNVMPQLHMPLQSGSDAVLRRMRRS YRKEKYLDIISRVRSAIPDAAITTDIIVGFPGETEDDFAETLDVVRKARFAGAFTFQYSK RPGTPAAEMPDQVPPDVVADRFARLVALVEQIALEENQAQVGRVVEVLVAEGEGRKDAET HRMSGRAPDNRLVHFRATDARPGDVVTVAVTQAAPHCLIADQVLGVRRTRAGDAWEARRS VRPSGVLLGMPALRPRT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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