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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP380436VDXYeast1mgQuote
EP380436VDXE. coli1mgQuote
BP380436VDXBaculovirus200ugQuote
MP380436VDXMammalian Cell200ugQuote

Protein Information

SpeciesVerminephrobacter eiseniae (strain EF01-2)
UniProt IDA1WIL9
Gene NamemiaB; Locus:Veis_1721
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMAKKVFIKTFGCQMNEYDSDKMADVLHAAQGYEPTQNVEEADLILFNTCSVREKAQEKVF SDLGRVKHLKARGVRIGVGGCVASQEGAEIIRRAPYVDVVFGPQTLHRLPELLQQRERLD QPQIDISFPEIEKFDHLPAASVQGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDDVL VEVAGLADQGVKEVTLLGQNVNAYRGRMGSTAQRADLALLLDYVAGIPGVERIRYTTSHP NEFTPRLIEAYARLPKLANHLHLPVQHGSDRILMAMKRGYTAMEYKSTVRKLRAIRPDLA LSSDFIVGFPGETQDDFDKLMRLIAEVHFDNSFSFVFSPRPGTPAAGLPDDTPHEVKLRR LQQLQGVIDTHIRSISASRVGTVQRILVEGASRRDAAELMGRTECNRVVNFAGPPHLVGQ MVELTITEARAYTLRGQYCAQESPQATMAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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