Request QuoteCatalog Number: xP380406AUCSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP380406AUCYeast1mgQuote
EP380406AUCE. coli1mgQuote
BP380406AUCBaculovirus200ugQuote
MP380406AUCMammalian Cell200ugQuote

Protein Information

SpeciesAcidovorax sp. (strain JS42)
UniProt IDA1WBW2
Gene NamemiaB; Locus:Ajs_3625
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-448
Expression Tag6xHis
Purity>90%
AA SequenceMSKKVFIKTFGCQMNEYDSDKMADVLGAAQGYEPTDDPEQADLILFNTCSVREKAQEKVF SDLGRVKHLKDKGVLIGVGGCVASQEGEEIIKRAPFVDVVFGPQTLHRLPELLNARAAKA RPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVL VEVAGLADQGVKEVTLLGQNVNAYLGAMGDTAEKADFALLLEYVAEIPGIERIRFTTSHP NEFTPRLIEAYAKIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDMA MSSDFIVGFPGETEEDFQKMMKLIDDVRFDNSFSFIFSPRPGTPAANLHDDTPHEVKLRR LQELQAVINRNILEISQERVGTVQRLLVEGVSKRDGSELMGRTECNRVVNFPGHERLIGQ MIDVKITEARTYTLRGEVVMGEVTTAVG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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