Request QuoteCatalog Number: xP386332STLSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP386332STLYeast1mgQuote
EP386332STLE. coli1mgQuote
BP386332STLBaculovirus200ugQuote
MP386332STLMammalian Cell200ugQuote

Protein Information

SpeciesShewanella baltica (strain OS155 / ATCC BAA-1091)
UniProt IDA3D7M8
Gene NamemiaB; Locus:Sbal_3261
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-474
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEASEADILLLNTCSIREKAQEKVF HQLGRWKTLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQVRR GEKAVIDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDD IILEIAQLAEQGVREVNLLGQNVNAYRGATHDGAICTFAELLRFVAAIDGIDRIRFTTSH PIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKAREGI QISSDFIIGFPGETKEDFADTIKLIEDIGFDHSFSFIYSARPGTPAADLPDNVDMEEKKQ RLAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQPKHIG TFVDVEIVDVYTNSLRGVFIRGEDEMDLRRSLRPAEILAKRKQDDELGVTQFKP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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