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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP397477ODJYeast1mgQuote
EP397477ODJE. coli1mgQuote
BP397477ODJBaculovirus200ugQuote
MP397477ODJMammalian Cell200ugQuote

Protein Information

SpeciesOrientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)
UniProt IDA5CC78
Gene NamemiaB; Locus:OTBS_0187
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-449
Expression Tag6xHis
Purity>90%
AA SequenceMNKMLYIKTYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYS ELGRIKKLQQSRLKAGKSKAKIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLE KLDSGTENHLIDLDFVEAAKFNKLPEVLKSPTVSGLVSVQEGCDKFCTFCVVPYTRGAEF SRPLEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRI RYITSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHTRERYLEIIQQLR ELRPDIVISSDIIVGFPGEDDEDFEATLSLAKEAKFGQCYSFKYSQRPGTPAAVKQQISE EVKQHRLSILQAQLMLQQLECNQKLIGKVVPVLFDRDGKYDGQIIGKTPYMQSVCIMNEK DNNLYGKIVNVKILSASASSLFGEVCPNS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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