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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP380283PYTYeast1mgQuote
EP380283PYTE. coli1mgQuote
BP380283PYTBaculovirus200ugQuote
MP380283PYTMammalian Cell200ugQuote

Protein Information

SpeciesPolaromonas naphthalenivorans (strain CJ2)
UniProt IDA1VSP2
Gene NamemiaB; Locus:Pnap_3373
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-447
Expression Tag6xHis
Purity>90%
AA SequenceMPKKVFIKTFGCQMNEYDSDKMSDVLHAAEGYENTQNIDEADLILFNTCSVREKAQEKVF SDLGRVKHLKAKGVLIGVGGCVASQEGEAIIQRAPYVDVVFGPQTLHRLPQLLASRARLG KPQVDISFPEIEKFDHLPPARVEGASAFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVL VEVAGLADQGVKEVTLLGQNVNAYRGSMGGTAEIADFATLLEYVSEIPGIERIRYITSHP NEFTQRLIDAYATLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLA MSSDFIVGFPGETEDDFEKLMKLVNDVGYDTSFSFIFSPRPGTPAANLADDTPHEVKLKR LQHLQATLDDSVRAISASRLGTVQRILVEGTARKDASELMGRTECNRVVILKGQPRLIGQ MVDVRITEASQRSLRGEVLTQDDTVAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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