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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP379308DHYYeast1mgQuote
EP379308DHYE. coli1mgQuote
BP379308DHYBaculovirus200ugQuote
MP379308DHYMammalian Cell200ugQuote

Protein Information

SpeciesDesulfovibrio vulgaris subsp. vulgaris (strain DP4)
UniProt IDA1VF04
Gene NamemiaB; Locus:Dvul_2004
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-449
Expression Tag6xHis
Purity>90%
AA SequenceMHDRTFHIETFGCQMNVNDSDWLARALMERGFSPAPFGEARLTIVNTCSVRDKPEQKVYS LLGRIRQATGKKPDAFVAVGGCVAQQIGSGFFSRFPQVRLVFGTDGLAMAPQALDRLVEE PDLKLSLLDFSEDYPERDAVLGQGAVPASVFVNIMQGCDNFCAYCIVPYTRGRQKSRATG TILDECRALLDRGAREITLLGQNVNSFGQDSHGDGTTFAQLLHKVAALPGLERLRFVTPH PKDIAPEVVEAFGTLPNLCPRLHLPLQAGSDRILKLMGRRYDMARYLRIVDDLRAARPDI VLSSDIIVGFPGETEEDFMETMGALETVGYAASYSFCYSDRPGTRAEMLPDKLSREVKLE RLERLQTLQNRLTERCLQDMVGKKVEVLLEGMSRKPGDEGDSWQGRDPYGNLVNVALPQG SDVRGRFLPVVVAQAKKHSLLAEQAGAPW
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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