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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP412490JAQYeast1mgQuote
EP412490JAQE. coli1mgQuote
BP412490JAQBaculovirus200ugQuote
MP412490JAQMammalian Cell200ugQuote

Protein Information

SpeciesJanthinobacterium sp. (strain Marseille) (Minibacterium massiliensis)
UniProt IDA6SV24
Gene NamemiaB; Locus:mma_0431
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-446
Expression Tag6xHis
Purity>90%
AA SequenceMQKKIYIKTFGCQMNEYDSDKMVDVLNASEGLIKTDTPEDADVILLNTCSVREKAQEKVF SDLGRLRELKFNNPDLVIGVGGCVASQEGDAIVKRAPYVDLVFGPQTLHRLPEMLKQRRS TGRSQVDISFPEIEKFDHMPPAKVEGATAFVSIMEGCSKYCSYCVVPYTRGEEVSRRFED VLAEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAELEGIERIRFVTSH PKEFTQRLIDAYAKVPKLVNHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLREVRPNI SISSDFIVGFPGETDADFEAMMKLINDIGYDNSFSFIFSPRPGTPAANLEDDTPHEVKLQ RLQRLQAVIDQNTRRYSDEMVGTVQRILVEGPSKKDPDELQGRTENNRVVNFAAGEHGAR LIGQMVDVNIVQSFAYTLRGEIIVKQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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