Request QuoteCatalog Number: xP369728MOISize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP369728MOIYeast1mgQuote
EP369728MOIE. coli1mgQuote
BP369728MOIBaculovirus200ugQuote
MP369728MOIMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium avium (strain 104)
UniProt IDA0QIR4
Gene NamemiaB; Locus:MAV_3625
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-715
Expression Tag6xHis
Purity>90%
AA SequenceMTCGFSRADRSPYHGPVTSTVARDVSGVRTYQVRTYGCQMNVHDSERLAGLLEAAGYRRA AEGAEVADVVVFNTCAVRENADNKLYGNLSHLAPRKRSNPQMQIAVGGCLAQKDREAVLR RAPWVDVVFGTHNIGSLPTLLERARHNKAAQVEIAEALQQFPSSLPSARESAYAAWVSIS VGCNNSCTFCIVPSLRGKEVDRSPDDILAEVRSLVADGVLEVTLLGQNVNAYGVSFADPA LPRDRGAFARLLRACGEIDGLERVRFTSPHPAEFTDDVIEAMAQTPNVCPALHMPLQSGS DRVLRAMRRSYRAERYLGIIDRVRAAMPHAAITTDLIVGFPGETEEDFAATLDVVRRARF AAAFTFQYSKRPGTPAAELDGQIPKAVVQERYERLVELQESISLQGNQALVGQTVELLVA TGEGRKDSATARMSGRARDGRLVHFAADDRVRPGDLVTTVITGAAPHHLIADAGILSHRR TRAGDAHAPAGARAASVSACPPSGRRRPGPTRWMCLMNDDRNHDDPRLGALRTEIEAAER RVAGGIDPGARGFVVSILVFVLLGSFILPHTGDVRGWDVLFGTHDAGAAAVALPSRVFGW LALVFGVGFSTLALVTRRWALAWIALAGTAIAGAAGMLAIWSRQTVPAGHPGPGWGLIVA WITVLVLIYQWARVVWSRTIVQLAAEEQRRRVAAQQQSTTLLDDLPKPEDPAAGT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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