Request QuoteCatalog Number: xP367816FDWSize: 0.2-1mg

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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP367816FDWYeast1mgQuote
EP367816FDWE. coli1mgQuote
BP367816FDWBaculovirus200ugQuote
MP367816FDWMammalian Cell200ugQuote

Protein Information

SpeciesFrancisella tularensis subsp. novicida (strain U112)
UniProt IDA0Q6T5
Gene NamemiaB; Locus:FTN_1063
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-442
Expression Tag6xHis
Purity>90%
AA SequenceMKEQKKVFIKTLGCQMNEYDSARMHEVLNEHFDTVKTDDYKDADIILINTCSIREKAQEK VFHELGRWKGLKKTNEDLIIGVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKQK QKTQQSQVDISFPEVEKFDYLPEPKAEGAKAYVSIMEGCDKYCSYCVVPYTRGPEVNRPF EDVLAECAILAEQGVKEITLLGQNVNHYLGPMENGQTADLALLIHFIAEIDGIERIRFTT SHPVEFSQNLIDAYATVPELANHLHLPVQHGSDRILINMKRNHTILEFKQKIRKLRAIRP DITISSDFIVGFPGETEEDFQKLLDLVKEINFDQSFSFIYSKRPGTPAADLPDDTPMEVK KDRLKRLQDLLNSNAQIISRQMVGTNQRILVDGTSKKDDNILSGRTENNRVVNFKGDKSL IGQFAMVKITESLPNSLRGELI
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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