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Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB

Recombinant (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP368496AUHYeast1mgQuote
EP368496AUHE. coli1mgQuote
BP368496AUHBaculovirus200ugQuote
MP368496AUHMammalian Cell200ugQuote

Protein Information

SpeciesAeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240)
UniProt IDA0KN81
Gene NamemiaB; Locus:AHA_3241
Protein Name (Dimethylallyl) adenosine tRNA methylthiotransferase MiaB
Region Expressed1-477
Expression Tag6xHis
Purity>90%
AA SequenceMSKKLHIKTWGCQMNEYDSSKMADLLDASNGYTLTEEPEEADVLLLNTCSIREKAQEKVF HQLGRWKKLKANKPGLVIGVGGCVASQEGDNIRTRAPYVDIVFGPQTLHRLPTMIKQVQE GRGAQVDVAFPEIEKFDSLPEPRAEGATAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD VLYEIAQLAQQGVREVNLLGQNVNAYRGPTFDGGICTFAELLRLVAAIDGIDRIRYTTSH PIEFTDDIIEVYKDTPEVVSFLHLPVQSGSDRILTMMKRPHTVLEYKSKIRRLRAARPDI TISSDFIVGFPNETDEDFEATMKLIEEINFDMSFSFIYSPRPGTPAADLPDDVDMEVKKA RLTRLQHVINNQSMQIGRAMLGSTQRILVEGPSKLDPMQLCGRTENNRVVNFEGPHTLIG GFADVEITEVRPNSLRGKFLRGENEMNLRIATAPSEILARRPDNVPDPLGVAAFTPH
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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